Microorganisms get introduced in operation theatres, NICU , PICU, ICCU due to many factors. These areas significantly contribute to nosocomial infections if not washed , cleaned and disinfected. Hospitals can pose a great risk to patients.
Swabbing of different sites and equipments in these critical areas helps to identify the aerobic or anaerobic bacterial or fungal colonization on these surfaces and equipments.
Isolation and Identification :
The swabs showing the growth are isolated on the nutritive and selective agar . The isolated colony is further processed to identify the organism . The contaminant can be normal flora or potential pathogen.
Initially organisms are classified into gram positive or gram negative.
Secondary and confirmatory tests are further carried out to identify the